Feulgen densitometry in image analysis.

Analysis of nuclear DNA content in Capsicum (Solanaceae) by flow cytometry and Feulgen densitometry.

Flow cytometric measurements of nuclear DNA content were performed using ethidium bromide as the DNA stain (internal standard, Hordeum vulgare 'Ditta', 1C = 5.063 pg) in 25 samples belonging to nine diploid species and four varieties of Capsicum: C. chacoense, C. parvifolium, C. frutescens, C. chinense, C. annuum var. annuum, C. baccatum var. baccatum, C. baccatum var. pendulum, C. baccatum var...

Feulgen densitometry: some observations relevant to best practice in quantitative nuclear DNA content determination

Despite of a huge amount of literature on the Feulgen reaction for DNA relatively few in­ vestigations deal with the special needs of plant scientists. As a consequence various mod­ ifications of the method are practised in different laboratories, which may in part be re­ sponsible for contradictory results. In the present work tests are conducted to find out, which steps of the procedure must ...

Flow Cytometric Analysis of Megakaryocyte Ploidy . Comparison With Feulgen

Flow cytometry (FCM) was adapted to measure ploidy values in megakaryocytes isolated from normal guinea pigs and African green monkeys. The FCM approach was validated by microscopic identification of electronically sorted SN and higher ploidy cells and by comparisons with Feulgen microdensitometry on the same samples. Sorted 8N populations contained only megakaryocytes and were completely free ...

Feulgen-dna Values in Megakaryocytes

Flow Cytometric Analysis of Megakaryocyte Ploidy . Comparison With Feulgen Microdensitometry

Flow cytometry (FCM) was adapted to measure ploidy values in megakaryocytes isolated from normal guinea pigs and African green monkeys. The FCM approach was validated by microscopic identification of electronically sorted SN and higher ploidy cells and by comparisons with Feulgen microdensitometry on the same samples. Sorted 8N populations contained only megakaryocytes and were completely free ...